c-Cbl, but not Cbl-b, expression is reduced in EM CD4 T cells. (A) Quantification of c-Cbl expression level in CM and EM cells by RPP arrays and immunodetection of Western blots (WB) with antibodies specific for c-Cbl and actin. Shown are c-Cbl to actin ratios in 10 (RPP arrays, 30 cells equivalent/spot) or 4 (WB, 300 000 cells equivalent/lane) distinct subjects. Examples of detected spots (B) and quantified immunodetections (C) are shown. (D) Quantification of Cbl-b by RPP arrays in 9 subjects as described for panel A.