CD40 rapidly ejects corepressors from the BCL6 repression complex on the CD23b promoter. (A) Chromatin immunoprecipitations were performed in Ramos cells at the indicated time points of CD40L exposure using BCL6 antibody. QPCR was performed to measure the enrichment of the CD23b promoter BCL6 binding site compared with input chromatin. The BCL6 enrichment values were first normalized to the input levels and expressed relative to untreated cells. The results correspond to the average of 3 independent replicates. (B) Chromatin immunoprecipitations were performed with SMRT and HDAC3 antibodies at the indicated time points after CD40L exposure to Ramos cells. The enrichment values for the different corepressors were first normalized to input levels and expressed relative to untreated cells. The results correspond to the average of 3 independent replicates. Error bars represent SEM.