CD40 signaling induces several BCL6 target genes in lymphoma cells and primary centroblasts. (A) Ramos cells were exposed to CD40L for 0, 0.5, 2, 8, or 24 hours, after which QPCR was performed to detect the mRNA abundance of BCL6, ATR, p53, and CCL3. The mRNA levels were first normalized by GAPDH levels and expressed as fold change relative to untreated cells. (B) Purified CD77⁺ centroblasts were exposed to CD40L for 0, 0.5, 2, 4, 8, or 24 hours after which QPCR was performed to detect the mRNA abundance of BCL6, ATR, CCL3, and CD23b. The mRNA levels were first normalized by GAPDH levels and shown as fold change relative to untreated cells. These experiments were performed in triplicate. Error bars represent SD.