American Society of Hematology

Figure 3

$Figure 3. High purity of leukocyte fractions was achieved by immunologic cell separations. The purity of all cell fractions was assessed by flow cytometry using side-forward scatter and antigenic characteristics. The fraction of granulocytes in the preisolation reference control whole blood was 72% (A). SSC indicates side scatter channel. (B) Shown is the enrichment of monocytes from 18% to 98% in the Lymphoprep MNC fraction by removal of cells positive for CD3, CD7, CD16, CD19, CD56, CD123, and CD235A. Monocytes were depleted down to 0.08% in the Lymphoprep RBC pellet (C). The purity of granulocyte fraction was 98% after removal of CD14-positive cells from the lower Polymorphprep layer, and the monocyte contamination of granulocyte preparation was only 0.3% (D). Flow cytometric histograms and the plotted mean percentage of analyzed cells are presented.$

High purity of leukocyte fractions was achieved by immunologic cell separations. The purity of all cell fractions was assessed by flow cytometry using side-forward scatter and antigenic characteristics. The fraction of granulocytes in the preisolation reference control whole blood was 72% (A). SSC indicates side scatter channel. (B) Shown is the enrichment of monocytes from 18% to 98% in the Lymphoprep MNC fraction by removal of cells positive for CD3, CD7, CD16, CD19, CD56, CD123, and CD235A. Monocytes were depleted down to 0.08% in the Lymphoprep RBC pellet (C). The purity of granulocyte fraction was 98% after removal of CD14-positive cells from the lower Polymorphprep layer, and the monocyte contamination of granulocyte preparation was only 0.3% (D). Flow cytometric histograms and the plotted mean percentage of analyzed cells are presented.

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