Granulocytes acquire TF expressed in monocytes. Immunoblotting of MNC lysates against YFP demonstrates that TF-YFP fusion protein is well expressed in nucleofected monocytes regardless of LPS stimulation (A). Representative immunoblot shows that TF-YFP fusion protein is acquired by granulocytes, isolated from whole blood reconstituted with TF-YFP–expressing monocytes. WB indicates Western Blot (B). The population of TF-YFP–positive monocytes was increased under LPS stimulation of whole blood, reconstituted with TF-YFP–expressing monocytes (C). The population of CD15 + granulocytes, acquired TF-YFP in whole blood reconstituted with TF-YFP–expressing monocytes, is increased by LPS stimulation (D). Plots summarize the mean percentage and MFIs of TF-YFP + cells from the total CD14 + MNC population (E) or TF-YFP + cells from the total CD15 + granulocyte population (F). Error bars represent SEM. *P < .05 compared with nontransfected monocytes or baseline conditions of granulocytes, respectively.