Intracellular distribution of TF-YFP fusion protein in monocytes and granulocytes. TF-YFP is evenly distributed throughout the membrane of nonstimulated monocytes and is also accumulated in the cytoplasm as a single speckle (panel A top). LPS stimulation enriches TF-YFP localization onto the monocyte membrane, which extends the protrusions (panel A middle). Myristylated YFP localized in the plasma membrane on monocytes (panel A bottom). TF-YFP fusion protein, acquired by granulocytes, localizes within cytoplasmic speckles (panel B top, middle). Granulocytes did not acquire myristylated YFP (panel B bottom). Draq5 indicates DNA fluorescent marker.