RvE1 does not directly compete with thromboxane receptor ligand: [³H]-SQ-29548–specific binding with recombinant TPα. HEK293 cells were transiently transfected with TPα. Twenty-four hours later, cells were collected, and [³H]-SQ-29548 binding was carried out. Transfected HEK293 cells (∼ 0.2 × 10⁶) were incubated with [³H]-SQ-29548 (∼ 4.5 nM) for 60 minutes at 4°C in the presence or absence of unlabeled SQ-29548 or RvE1-free acid (0.1 to 1000 nM). Bound and unbound radioligands were separated by filtration and specific binding was determined. Results are means plus or minus SEM from duplicates of n = 3.