Representative images of thrombus growth versus time after ferric chloride–induced injury. Thrombus formation was induced with 10% FeCl₃ for 5 minutes in mesenteric arterioles, and platelet accumulation was analyzed for 45 minutes from the time of filter paper placement. Images of thrombus growth were taken every minute alternatively in transmitted and fluorescent light. VWF^−/− mice were injected with 50 μg of WT mVwf cDNA and mVwf cDNA mutated in the RGD sequence (D2509G), in the collagen-binding site (D1742A, S1783A, H1786A), or in the GPIb-binding site (K1362A). Original magnification, ×100.