Effect of WT and mutated mVWF on ferric chloride–induced injury in mesenteric arterioles of VWF^−/− mice after hydrodynamic injection. VWF^−/− mice were injected with 50 μg of WT pLIVE-mVwf or pLIVE-mVwf mutated in the RGD sequence (D2509G), in the collagen-binding site (D1742A, S1783A, H1786A), or in the GPIb-binding site (K1362A). Thrombus formation was examined 3 to 4 days after injection. Endothelial injury was induced with 10% FeCl₃ in mesenteric vessels 100 to 130 μm in diameter. When the vessel did not occlude within 45 minutes, observation was stopped. Each symbol represents one mouse, and the median value of each group is indicated. (A) Time required for the formation of the first thrombus greater than 30 μm in diameter. (B) Time required for the formation of an occlusive thrombus (cessation of blood flow).