Role of Dyn and Rab proteins on chemokine-induced D6 up-regulation on cell membrane and scavenging activity. CHO-K1/D6 cells were transiently transfected with the indicated pEGFP-tagged constructs. (A) Quantification of D6 membrane expression expressed as percentage of MFI of each transfected cell population treated with CCL3L1 (100 nM, 30 minutes) over untreated controls. Results (means ± SEM) are from at least 3 independent experiments. CCL4 degradation rate of sorted transfectants is shown in panels B (□ indicates pEGFP-N1; ■, Dyn-K44A; and ♦, Rab5-S34N), C (□ indicates pEGFP-N1; ○, Rab4-Q67L; and ●, Rab4-S22N), and D (□ indicates pEGFP-N1; △, Rab11-Q70L; and ▲, Rab11-S25N). Data were analyzed as described in “Chemokine scavenging assay.” Results (means ± SEM) shown in panel A are from at least 3 different experiments; results in panels B through D are from triplicates of 1 representative experiment of at least 3 performed. Asterisks indicate significant differences of Dyn/Rab-transfected versus pEGFP-N1–transfected cells (*P < .05; **P < .01).