Myelopoiesis in CBFβ-SMMHC/FLT3-ITD mice. (A) Representative peripheral blood smears from 2-month posttransplantation Bex/Vex (control) or CBFβ-SMMHC/FLT3-ITD mice indicating a high percentage of immature myeloid blasts in CBFβ-SMMHC/FLT3-ITD mice (original magnification ×500). (B) Wright-Giemsa staining of FACS–sorted Bex⁺/Vex⁺ cells from bone marrow of control or CBFβ-SMMHC/FLT3-ITD mice at 2 months after transplant. (C) Bex⁺/Vex⁺ cells were sorted from bone marrow or peripheral blood of CBFβ-SMMHC/FLT3-ITD mice at 2.5 weeks after transplant and cytospun onto glass slides for Wright-Giemsa staining (n = 3). A peripheral blood smear from the same animal used for isolation of FACS-sorted cells is shown at right.