Proportion of memory CD8⁺ T cells in the BM. (A) Ratio of CD4⁺ T cells and CD8⁺ T cells in paired blood and BM samples (n = 7). (B) Flow cytometric profiles showing the gating of memory CD8⁺ T cells. After gating on CD8⁺ T cells, the dual expression of CD45RA and CCR7 was used to identify memory T-cell subsets. CD8⁺ T cells that were CCR7⁺CD45RA⁺ were considered naive T cells, CCR7⁺CD45RA⁻ were central memory cells, CCR7⁻CD45RA⁻ were effector memory cells, and CCR7⁻CD45RA⁺ were effector memory cells with RA phenotype. Percentages represent proportion of each subset within CD8⁺ T-cell population. (C) Based on the expression of CD45RA and CCR7, the proportion of naive and memory CD8⁺ T cells were compared between paired blood and BM samples (n = 14). Line represents the median. (D) Dual expression of CD27 and CD28 were compared on effector memory CD8⁺ T cells from paired blood and BM samples. Percentages represent proportion of each subset within CD8⁺ T-cell population. (E) The differentiation status of effector memory CD8⁺ T cells from blood and BM were compared. Table shows the proportion of effector memory CD8⁺ T cells at various stages of differentiation in 3 paired blood and BM samples—HM101, HM102, and HM103.