CD8⁺ T cells from the BM have a unique homing phenotype. (A) Example flow cytometry dual expression profiles showing the staining for CD62L, CCR7, CXCR3, CCR5, CXCR6, and CXCR4 on CD8⁺ T cells in paired blood and BM samples. Percentages represent proportion of tetramer⁺ CD8⁺ T cells expressing the chemokine receptor. (B) Frequency of CD8⁺ T cells expressing CD62L, CCR7, CXCR3, CCR5, CXCR6, and CXCR4 in paired blood and BM samples were analyzed in 5 different subjects. (C) In a transwell migration assay, the percentage of CD8⁺ T cells that migrated to CCL5 and CXCL16 were estimated. CD8⁺ T cells from paired blood and BM samples were added to wells, and the proportion of the CD8⁺ T cells that migrated to CCL5 and CXCL16 were then analyzed. Statistical values were obtained by Wilcoxon signed rank test. The y axes show percentage of CD8⁺ T cells that migrated in response to the chemokine.