Chemokine profiles of virus-specific CD8⁺ T cells in paired blood and BM samples. (A) Example flow cytometry dual expression profiles showing the staining for CD62L, CCR7, CXCR3, CCR5, CXCR6, and CXCR4 in paired blood and BM samples on EBV-specific CD8⁺ T cells. After gating on CD8⁺ T cells, the expression of homing markers were identified in tetramer-positive T cells. The percentages shown refer to the percentage of tetramer-positive CD8⁺ T cells that express the relevant marker. (B) The summary of the frequency of antigen-specific CD8⁺ T cells expressing different homing markers. Graphs show the analysis of virus-specific CD8⁺ T cells from paired blood and BM samples for the expression of CD62L, CCR7, CXCR3, CCR5, CXCR6, and CXCR4 by flow cytometry. After gating on CD8⁺ T cells, the expression profiles of EBV- and CMV-specific CD8⁺ T cells were analyzed (n = 5-7). *Significant differences; P ≤ .05.