Figure 3
![Figure 3. Pulse-chase analysis of PZ and FX secretion. HEK 293 cells transiently transfected with expression vectors were divided into 5 dishes and were labeled with a 50 μCi [35S]protein labeling mixture for 1 hour. The chase was then initiated by changing the medium containing Vit.K, and the samples were incubated for 1, 2, 4, and 8 hours. PZ (A) or FX (B) in the medium (●) and in the cell lysates (○) were collected by immunoprecipitation using an anti-PZ or anti-FX antibody. Their intensity under fluorography was calculated by setting the signal in the cell lysates at chase-time 0 to 100%. The data shown are the mean (± SD) for 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/16/10.1182_blood-2008-07-171884/7/zh80170934010003.jpeg?Expires=1736057618&Signature=Q1aEVEMiqayiFF9~x3VBdITjh52L9xrCtFsI45emj4fjDU9Xs4DBYCIrqA5mCre0E4CtuX6FiWsxGfDUHMMBkrzdR~sJ6mbpkHv4UqMIIVdbhulzIjgH062GX3uMtA5noQKVxatvr9K87zr~3etyzdRjby6VIDSplkhRwHYR2VTulIlXt~SjKszDwX8Dr9O3T8hhT~iBh5e7PtvpUGke5hQVRVnouw7smszf64ehyehpo4GIJY3qBdskuw4woWEXsTP4ZdenKNiYt3xla1qkslSx~ilY2Hgc52xnHOe5MUFtrufLYv5QWQ8vkVHqLNHOr9Y2rkLwd~mn-sY-SRghGg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of PZ and FX secretion. HEK 293 cells transiently transfected with expression vectors were divided into 5 dishes and were labeled with a 50 μCi [35S]protein labeling mixture for 1 hour. The chase was then initiated by changing the medium containing Vit.K, and the samples were incubated for 1, 2, 4, and 8 hours. PZ (A) or FX (B) in the medium (●) and in the cell lysates (○) were collected by immunoprecipitation using an anti-PZ or anti-FX antibody. Their intensity under fluorography was calculated by setting the signal in the cell lysates at chase-time 0 to 100%. The data shown are the mean (± SD) for 3 independent experiments.
