Increased recruitment and activation of host NK cells in lymph nodes after allogeneic DC immunization. B6 CD8^−/− mice were left untreated (−) or injected with either syngeneic B6 DCs or allogeneic BALB/c DCs. Twenty-four hours after immunization, draining lymph nodes were harvested, and the percentage of TCR-β^neg NK1.1^pos cells was determined (A) as well as the percentage of NK cells (TCR-β^neg NK1.1^posDX5^pos) expressing CD69 (B) and CD11b (C). (D) The frequency of CD69^pos NK cells (TCR-β^neg NK1.1^pos) producing IFN-γ was evaluated by ex vivo intracytoplasmic staining without prior stimulation. Data are mean plus or minus SEM of 4 or 5 mice per group. *P < .05. **P < .01. N.S. indicates not significant. Data are from one representative experiment of 3 performed.