Recently recruited NK cells are responsible for allogeneic DC killing. (A-C) B6 CD8^−/− mice, treated, or not, with anti-CD62L Mel-14 mAb, were immunized with CFSE^high syngeneic B6 DCs and CFSE^low allogeneic BALB/c DCs. Forty-eight hours after injection, draining lymph nodes were removed and analyzed for the presence of NK cells and CFSE DCs (CD11c^pos MHC II^high CFSE^pos cells). (A) Absolute numbers of NK cells (TCR-β^neg NK1.1^pos) recovered from lymph node are indicated. (B) Expression of CD127 and Ly49D on NK cells (TCR-β^neg NK1.1^pos). (C) Representative CFSE profiles of injected DCs from control, Mel-14–, or PK136-treated mouse lymph nodes are shown. Numbers indicate the percentage of CFSE^low BALB/c DCs among control CFSE^high B6 DCs. A representative experiment of 3 is shown.