miR-130a also antagonizes the antiangiogenic activity of HOXA5. (A) miR-130a antagonizes the antiangiogenic activity of HOXA5. HUVECs were cotransfected with either empty vector or pcDNA3.1-miR-130a and pcDNA3.1-HOXA5 and then incubated overnight before being subjected to tube formation assays. The ratio of cotransfection was 2:1 miR-130a:HOXA5 expression construct. Tube counts were determined as described in “Migration and tube formation assays.” (B) Quantification of tubes/LPF (*P < .01). Error bars represent SEM.