Effects of Ik6 overexpression on gene expression during erythroid differentiation. RT-PCR was performed using total RNA extracted from cultured cells derived from CB CD34⁺ cells. Gene expression was normalized with GAPDH expression. (A) Relative expression of erythroid genes (GATA1, EKLF, EPOR, NFE2, FOG1, and and α-globin) on days 17 and 20 between untransduced (CTRL) erythroid cells (as reference) and transduced cells. Expression of these genes was reduced in EF1 IK6 erythroid cells. The unchanged results on days 10 and 13 are not shown. (B) Relative expression of myeloid genes (PU1, M-CSFR, and CD13) on days 17 and 20 between untransduced (CTRL) cells (as reference) and transduced cells. Expression of these genes was increased in EF1 IK6 erythroid cells. The unchanged results on day 10 are not shown, and the results on day 13 were similar to that of day 17. Data are mean values (± SEM) for 4 to 7 experiments. *P < .05 using the Wilcoxon test when comparing with CTRL and EF1 GFP conditions; n.s. indicates not significant.