FACS analysis of ET-BR surface expression in THP-1 and PBM. (A,C) THP-1 cells and PBM were treated with PlGF for 24 hours and surface expression of ET-BR was analyzed by flow cytometry. (B,D) The mean fluorescence intensity of ET-BR in PlGF treated THP-1 cells and PBM in the presence or absence of indicated pharmacologic inhibitors is shown as a histogram. Data are representative of 3 independent experiments in duplicates.