Figure 1
Validation of ChIP-chip–identified SALL4-binding sites by Q-RT-PCR. Chromatin immunoprecipitation experiments were performed in NB4 cells with antibodies raised against SALL4. Forty-three randomly selected promoters identified as SALL4 targets with ChIP-chip arrays were analyzed by Q-RT-PCR. ChIP-Q-RT-PCR identified 39 targets having more than 2-fold enrichment versus the input control. The fold change of SALL4 immunoprecipitated DNA over the input is presented on a log2 scale. Negative control primers were designed based on the genomic regions surrounding the peak as shown in Figure S2.
