SALL4 binds and transcriptionally regulates genes involving various apoptotic pathways. (A) SALL4 binds to apoptosis-inducing genes, apoptosis-inhibiting genes, as well as genes involved in other apoptosis processes based on the PANTHER classification system.34 (B) Q-RT-PCR shows differentially regulated expression levels of apoptotic genes in SALL4 knockdown NB4 cells. Error bars represent standard deviations for duplicate measurements (quantification replicas) normalized to GAPDH levels. Lowering levels of SALL4 expression in NB4 cells induces gene expression that favors apoptosis. (C) When comparing the SALL4 target gene set with the total analyzed gene pool, we found that SALL4 binds to genes involving various apoptotic pathways, including p53, BCL2, TNF, and PTEN. Data were processed using Ingenuity Pathway Analysis.
