Transcriptome analysis of MCL1 overexpressing B-NHL cells. (A) Gene-expression profile of MCL1-overexpressing B-cell lymphomas. Gene-expression profiling was performed in duplicate for MCL1 overexpressing RAMOS RA-1 vs empty vector RAMOS RA-1, as well as MCL1 overexpressing Z-138 vs empty vector Z-138. The heat map represents expression levels for each sample. A total of 209 top significant genes (Log₂ (Cy5/ Cy3) cut-off = 1) were ranked by Euclidean squared functions using GEPAS and BABELOMICS tools (http://babelomics.bioinfo.cipf.es/). Genes in blue denote genes that were found cross-validated in human follicular B-cell lymphomas (Figure 5). (B) Gene ontology analysis for the 316 annotated human genes commonly up- or down-regulated by MCL1 overexpression (Log₂ (Cy5/ Cy3) cut-off = 0.5) was performed with the PANTHER program. Categories with more than 25 genes in biologic processes are shown. (C) Gene ontology analysis of the genes regulated by MCL1 overexpression was done with the PANTHER program. Of the 468 human genes showing a Log₂ (Cy5/ Cy3) cut-off = 0.5 commonly up- or down-regulated by MCL1 overexpression, 316 were assigned with multiple molecular functions. Proteins for which no molecular function could be assigned were omitted from this display. Categories with more than 20 assigned proteins are shown. Subdivisions of the category transcription factor (46 proteins) is also shown.