Figure 2
Figure 2. Cleavage of immunotoxins by lysosomal proteases. The immunotoxin B3(dsFv)-PE38 was incubated with lysosomal extract from Raji cells or with one of the purified lysosomal proteases cathepsin B, cathepsin D, or cathepsin S. Samples of the reaction were removed immediately after the addition of enzyme (0) and at various time intervals up to 60 hours after the start of the incubation, then analyzed by reducing SDS-PAGE. Arrows indicate the VH and VL-PE38 polypeptides that compose the mature immunotoxin and the lysosomal protease cleavage fragments sequenced by Edman degradation.

Cleavage of immunotoxins by lysosomal proteases. The immunotoxin B3(dsFv)-PE38 was incubated with lysosomal extract from Raji cells or with one of the purified lysosomal proteases cathepsin B, cathepsin D, or cathepsin S. Samples of the reaction were removed immediately after the addition of enzyme (0) and at various time intervals up to 60 hours after the start of the incubation, then analyzed by reducing SDS-PAGE. Arrows indicate the VH and VL-PE38 polypeptides that compose the mature immunotoxin and the lysosomal protease cleavage fragments sequenced by Edman degradation.

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