Electrophoresis of PCR products of proband, parents and control. Upper bands correspond to PCR products obtained by using gap-F and gap-R primers designed by Le Gac et al.³  Lower bands correspond to HFE exon 5. Lane 1: DNA molecular marker II; lane 2: proband; lane 3: father; lane 4: mother; lane 5: control; lane 6: negative; and lane 7: DNA molecular marker VIII (Roche Diagnostics, Mannheim, Germany).