Altered Vβ usage of heterologous immune T-cell responses in LCMV-immune HP mice. LCMV-immune HP or WT mice were challenged with PV, and 7 days later splenocytes were stained with NP-205 tetramer and with the entire panel (Vβ2-14) of mAb to Vβs. (A) A linear regression analysis of the number of NP-205⁺Vβ5.1/5.2⁺ costaining cells (y-axis) versus the number of NP-205⁺ cells (x-axis). (B) The NP-205 responses in individual HP mice () and the proportions of those cells staining with mAb to Vβ5.1/5.2 () or to no tested Vβ (Vβ-others; ▬). (C) A linear regression analysis of WT mice NP-205 responses to Vβ-others, and (D) the responses of individual WT mouse to Vβ5.1/5.2 and Vβ-others, as in panel B. These data are a composite of 2 different experiments.