Expansion of T_Reg (CD4/CD25^Hi cells) following coincubation with Tregitope. The expansion of CD4⁺CD25^Hi T cells among PBMC restimulated with dust mite lysate following primary incubation of PBMC with either dust mite lysate alone or dust mite lysate and hTregitope 289, or dust mite lysate and hTregitope 167. FoxP3 was not assessed. Numbers in boxes are the percentage of cells in that gate.