Schematic representation of 6 constructs used to investigate the specificity of quinine-dependent antibodies recognizing GPIIIa in Western blot. (Left) Schematic representation of GPIIb/IIIa based on crystallographic studies of alpha_v/beta₃¹⁵  and alpha_IIb/beta₃¹⁶  integrins. Adapted from Humphrey and Mould,¹⁷  with permission. Ovals indicate structural domains incorporated into constructs 1 to 3. (Right) Diagram indicating amino acid sequences of constructs 1 to 3 and mutations incorporated into construct 1 to produce constructs 4 to 6. V5 and HIS recognition sites are described in “Preparation of cDNAs encoding truncated mutant versions of GPIIIa.”