Liar interacts with Lyn. (A) Lyn and Liar coimmunoprecipitate. Western blot analysis of Lyn and Liar immunoprecipitates (IP) from COS-7 cells transfected with Lyn and myc-tagged Liar. Blots were probed with anti-Lyn and anti-myc antibodies. (B) Lyn-Liar coimmunoprecipitation is not dependent upon kinase activity. Western blot analysis of Lyn immunoprecipitates (IP) from COS-7 cells transfected with Lyn wild-type (LynWT), kinase inactive (LynY397F), constitutively active (LynY508F), and myc-tagged Liar. Blots were probed with anti-Lyn and anti-myc antibodies. (C) Liar and Lyn interact directly. GST pull-down assays using purified GST/GST-Lyn and in vitro–translated [³⁵S]-radiolabeled Liar. Radiolabeled Liar was detected on Western blot analyses using a PhosphorImager, then probed with anti-Lyn and anti-GST antibodies. (D) Overlapping subcellular localization of Liar and Lyn. COS-7 cells transfected with Lyn and myc-tagged Liar were analyzed by confocal microscopy and immunofluorescence staining using anti-myc and anti-Lyn antibodies.