Liar, Lyn, and HS1 interaction analysis. (A) Three of the 4 ankyrin repeats of Liar bind Lyn. Full-length Liar and deletion mutants, were tested for interaction with Lyn in a yeast 2-hybrid assay. (B) The SH3 domain of Lyn binds Liar. Full-length Lyn and its domains (Un-unique, SH3, SH2, and kinase) were tested for interaction with Liar in a yeast 2-hybrid assay. (C) Liar, Lyn, and HS1 coimmunoprecipitated. COS-7 cells transfected with Lyn, HS1, and myc-tagged Liar were analyzed by Western blot analysis after immunoprecipitation (IP) of Liar. Blots were probed with anti-Lyn, and-HS1 and anti-myc antibodies. (D) Liar, Lyn, and HS1 form a complex in erythroid cells. Liar was immunoprecipitated (IP) from J2E cells, compared with nonspecific IgG immunoprecipitates. Blots were probed with anti-Lyn, anti-Liar, and anti-HS1 antibodies.