Ectopic expression of Pim-1 enhances the resistance of v-Abl–transformed cells to apoptosis induced by imatinib. (A) Apoptosis and cell survival were examined as described in Figure 5. Shown are wild-type (WT) cells and Pim-1^−/−/Pim-2^−/−/Pim-3^−/− (TKO) cells ectopically expressing either wild-type Pim-1 (TKO/Pim1), kinase-death Pim1 (TKO/Pim1KD), or empty vector (TKO/Empty). Plotted are the results from 3 independent experiments. Error bars represent SD. (B) An immunoblot of v-Abl–transformed WT cells, TKO cells, and TKO cells expressing different proteins was probed with indicated antibodies.