Pim mediates phosphorylation of Ser112 of BAD in v-Abl–transformed cells. Shown is a blot probed as indicated. Lane1, wild-type cells; 2, Pim-1−/−/Pim-2−/−/Pim-3−/− (TKO) cells; 3, TKO cells expressing Pim-1; 4, TKO cells expressing Pim-1 kinase-dead (Pim-1KD).