Hematopoietic reconstitution from mKL cells grafted in RAG1^−/− C57BL/6 (Ly5.1) mice. (A) A mean of 2 × 10⁴ AFKL cells sorted from GFP⁺ Ly5.2⁺ embryos were transplanted in sublethally irradiated RAG1^−/− C57BL/6 (Ly5.1) mice. As a positive control, 2 × 10⁴ FLKL cells were injected in the same model (Table 1). Representative flow cytometry profiles of BM, spleen, and lymph nodes of RAG1^−/− C57BL/6 (Ly5.1) transplanted mice 16 weeks after transplantation (demonstrating multilineage reconstitution with B lymphoid, T lymphoid, and myeloid cells) are shown. (B) Representative flow cytometry profiles of BM of RAG1^−/− C57BL/6 (Ly5.1) transplanted mice showing multilineage reconstitution with B lymphoid and myeloid cells 16 weeks after secondary transplantation. Dot plots are shown after gating for the GFP⁺ Ly5.2⁺ fraction. Two recipients of the 7 transplanted engrafted. These 2 recipients were transplanted with different KL samples: one not sorted (1.5 × 10⁷ cells) and the other sorted for Ly5.2 expression (1.5 × 10⁴ cells) before transplantation.