Frequency of expression of hematopoietic genes and their coexpression in mFLKL, mAFKL, and mAmKL cells. (A) Single-cell multiplex RT-PCR analyses were performed to investigate the frequency of expression of hematopoietic genes in the 3 cell populations. In all cases 28S was used as an endogenous control. The results are shown as histograms representing the proportion of cells expressing each gene. (B) Single KL cells were sorted and analyzed by multiplex RT-PCR, as described in “Methods.” Each row shows the same (numbered) individual cell. Each column shows a different gene. Empty symbols represent cells not expressing that particular mRNA (< 2 mRNA molecules); gray symbols, positive cells where mRNA levels were not quantified.