T-cell disease is oligoclonal. Analysis of TCR-β rearrangement. Thymocyte/tumor cDNA was amplified using primer pairs specific for each of the 19 and 20 possible variable/constant region junctions at the murine TCR-β locus (A) and TCR-α (B) locus, respectively. The samples were electrophoresed through a 1.8% agarose gel and stained with ethidium bromide. Top line represents KM donor; middle and bottom lines, 2 different tumor samples.