Chemokine scavenging function of murine erythrocytes. (A) A total of 108 erythrocytes with differing storage times were incubated with 2 nM CCL2. CCL2 concentrations in supernatant increased with RBC storage time (P < .001). (B) A total of 108 WT or KO erythrocytes were incubated with 2 nM CCL2. For chemokine incubated with WT erythrocytes, CCL2 concentrations increase in the supernatant and decrease in the lysate over time, (+ indicates P = .006 and P = .011 for WT supernatant and lysates, respectively; P was not significant for KO). There were significant differences in CCL2 between WT and KO erythrocytes (* indicates P = .027 and P = .012 for supernatatant from 11- and 21-day-old erythrocytes; P = .057 from 17-day-old erythrocytes; lysates P < .001 for all days). (C) A total of 108 WT or KO erythrocytes were incubated with 2 nM MIP-2 for 30 minutes. (P < .001 for WT erythrocytes and P = .102 for KO erythrocytes). There were significant differences in supernatant MIP-2 between WT and KO erythrocytes that were stored for 1, 2, 9, or 12 days (P = .002, P = .019, P < .001, and P = .002, respectively), but not when erythrocytes were stored for 16, 23, or 26 days (P = .091, P = .200, P = .642, respectively).
