SHD1 is induced by STAT5-activating cytokines. (A) BaF3/EpoR cells were starved overnight, and then stimulated with 10 U/mL Epo for the indicated times. The expression of SHD1 was analyzed by Northern blotting. (B) Murine splenic T cells were expanded as described in “T-cell proliferation and stimulation assays.” The cells were starved, and then stimulated with IL-2 (100 U/mL) for the indicated times. A indicates asynchronously growing cells. (C) 32D cells growing in the media containing IL-3 were washed with PBS, and immediately transferred into the media containing 10 ng/mL IL-3 or 50 ng/mL G-CSF. The cells were harvested at the indicated time points, and the expression of SHD1 was analyzed by Northern blotting.