5-LO and FLAP mRNA expression in MNC, PBM, and THP-1. (A,B) qRT-PCR analysis of mRNA in MNC isolated from SCD subjects at steady state (n = 9) and healthy controls (n = 9). Each data point represents ΔC_t values of 5-LO and FLAP expression from individual subjects. Mean values are represented as black bars. Error bars represent SEM. (C) ΔΔC_t (RQ) values show fold expression of 5-LO and FLAP mRNA in SCD compared with healthy controls. (D) RPA analysis of FLAP, HIF-1α, and GAPDH in PlGF-treated THP-1 cells and PBM for the indicated time periods. Data are representative of 3 independent experiments. Where indicated, the vertical lines show repositioned gel lanes.