PlGF increases FLAP and HIF-1α protein in THP-1 cells. THP-1 cells were pretreated for 30 minutes with LY294002 and DPI followed by PlGF treatment for indicated time periods. (A) Cytosolic proteins were subjected to western blot analysis using antibody to FLAP. The same membrane was reprobed with β-actin antibody to normalize protein loading. (B) Nuclear extracts were subjected to western blot analysis using antibody to HIF-1α. The same membrane was reprobed with HIF-1β antibody to normalize the protein loading. Proteins were visualized by enhanced chemiluminescence corresponding to their expected molecular weights: FLAP (18 kDa), β-actin (42 kDa), HIF-1α (120 kDa), and HIF-1β (95 kDa). Data are representative of 3 independent experiments.