PlGF-mediated FLAP and LTE₄ expression involves HIF-1α. THP-1 cells were transfected with indicated siRNA constructs or expression plasmids, followed by PlGF treatment for 24 hours. (A) RPA, (B) LTE₄ release, and (C) Western blot analysis of nuclear extract using HIF-1α antibody. (D) Western blot analysis of cytosolic extracts from THP-1 cells treated with PlGF for indicated time period (6-24 hours) using PHD-2 antibody. Data are representative of 3 independent experiments. Data are expressed as means plus or minus SEM of 3 independent experiments (***P < .001; **P < .01; ns, P > .05). Where indicated, the vertical lines show repositioned gel lanes.