Analysis of Bcl11a-targeting CIS. (A) The schematic representation of the CIS-targeting Bcl11a. The solid arrows indicate the relative position and orientation of isolated proviral insertions at Evi9; the open arrows indicate the relative position and transcriptional orientation of Bcl11a and 3 other predicted genes; bracketed numbers, size (in kb) of genes. (B) Expression in BXH-2 leukemia of Bcl11a and 3 other predicted genes nearby Evi9 locus (Evi9-PG1, Evi9-PG2, and Evi9-PG3) was detected by RT-PCR using gene-specific primers. The lanes marked “−” indicate AML samples with no Evi9 insertions; the lane marked “+” indicates 1 of the 5 AML samples with Evi9 insertions. Gapdh was used as a loading control. NC indicates water in place of cDNA as the template for PCR was set up as negative controls.