Attenuation of experimental autoimmune hepatitis by infusion with LRDCs. (A) Schematic diagram of the induction and intervention of EAH. In the control, EAH was induced by double immunizations with liver S100 antigen in CFA. LRDC infusion was accomplished intraperitoneally 3 times in weekly intervals. Livers and blood were harvested for analysis 21 days after EAH induction. (B) Mean level of serum ALT in all groups (n = 6) was assayed. (C) Representative hematoxylin-eosin (HE) histologic observations of EAH lesions in animal liver after standard EAH induction. CoolSNAP cf camera (Roper Scientific Photometrics) mounted on a microscope (Leica-DMIRB) and Meta Imaging Series 5.0 (Molecular Devices) were used to acquire images. Arrow shows the characteristic periportal infiltrates in comparison with the minimal histologic lesions observed in EAH animals protected by LRDCs. Original magnification, 20×. (D) Liver draining lymph node cells isolated from the S100-immunized mice or LRDC/cDC-transferred mice were restimulated with S100 antigen or OVA antigen in vitro; the IFN-γ levels in the supernatant were then assayed by ELISA. (E) Turnover of T cells in the liver was measured by BrdU labeling. BrdU was administrated to mice via drinking water and intraperitoneal injection, and the liver NPCs were then prepared and assayed using FITC-labeled anti-BrdU antibody by FACS. Data represent the mean plus or minus SD of triplicate samples and are representative of 2 independent experiments. **P < .01.