Panning of PhD 7-phage peptide library. (A) Positive clonal analysis for phages following the third round of panning. Clones were subjected to ELISA for binding to anti–GPIIIa49-66 Ab. BSA is used as control antigen. Clones that gave an anti–GPIIIa49-66 Ab/BSA OD ratio more than 3 were designated positive. Sequence analysis is given for positive peptides analyzed by BLAST algorithm of the Blast program and database of the National Center for Biotechnology Information (NCBI). Three clones are molecular mimics with close sequence similarity to HCV proteins. PHC09 is a nonconserved peptide in the core-envelope 1 region. (B) Anti–GPIIIa49-66 reactivity with various 10-mer peptides: GPIIIa49-66, PHC09, homologous peptides (H1-H6) of PHC09, HIV-nef, and irrelevant peptide (IR). (C) Anti–GPIIIa49-66 reactivity with various 8-mer peptides. SEM is given. HCV genome map is provided.