Stem-cell fractions in JAM-A⁺ and JAM-A⁻ cells in BM. Stem-cell fractions (Sca-1⁺c-Kit⁺) in whole lineage-negative cells (left), lineage-negative JAM-A⁺ cells (middle), and lineage-negative JAM-A⁻ cells (right) in 1-week-old BM (A), 8- to 12-week-old BM (B), and 36- to 40-week-old BM (C). Each enclosure represents the percentage of enriched stem-cell fraction that is Sca-1⁺c-Kit⁺ in lineage-negative cells (A-C). Sca-1⁺c-Kit⁺ in whole cells (left), JAM-A⁺ cells (middle), and JAM-A⁻ cells (right) in 8- to 12-week-old BM. Enclosures represent the percentages of Sca-1⁺c-Kit⁺ in cells that are without lineage depletion procedure (D). Representative data from more than 3 independent experiments are shown.