Colony-forming activity of JAM-A⁺ and JAM-A⁻ cells. Colony-forming activity of sorted cells from fetal and adult tissues was examined. (A) CFU-Mix activity of 800 JAM-A⁺ cells versus 800 JAM-A⁻ cells in CD34⁺c-Kit⁺ cells in E11.5 FL and KSL cells in E14.5, E16.5, and E18.5 FL. (B) CFU-mix activity of 100 JAM-A⁺ cells without lineage depletion versus 100 KSL cells (i). CFU-mix activity of 100 JAM-A⁺ cells versus 100 JAM-A⁻ cells in KSL cells in 8- to 12-week-old BM (ii). Results from more than 3 independent experiments are shown. Error bars indicate SEM; *P < .05, **P < .001, #P > .2.