Effect of panobinostat on apoptosis regulators and apoptosis in cultured CTCL cells. (A) HH, HuT78, and MJ cells were treated with the indicated concentrations of panobinostat for 48 hours. Then, apoptosis was determined by flow cytometry. Columns represent mean of 3 experiments; bars, SEM. Inset: Representative Western blot of PARP cleavage from HH cells. (B) HH and MJ cells were treated with the indicated concentrations of panobinostat for 16 hours. Following this, cell lysates were prepared and immunoblot analysis was done for Bcl-2, Bcl-XL, Mcl-1, Bax, Bak, Bim, and XIAP. The levels of β-actin in the lysates served as the loading control. (C) HH and MJ cells were treated with the indicated concentrations of panobinostat for 16 hours. Following this, total cell lysates were prepared and immunoblot analysis was done for tyrosine-phosphorylated STAT1, STAT3, and STAT5. Blots were stripped and reprobed for total levels of STAT1, STAT3, and STAT5. The expression of β-actin in the lysates served as the loading control.
