Figure 2
Figure 2. Gene expression profiles of mouse Clec9A. Real-time PCR was used to determine the mRNA expression of the Clec9A gene relative to Gapdh using primers designed from the 3′ UTR region of mClec9A. (A) Expression in lymphoid organ steady state DCs including splenic cDC subsets (DN, CD4+, CD8+), thymic cDC subsets (CD8−, CD8+) LN cDC subsets (CD8−, CD8+), dermal and Langerhans cells (LC), and in thymic and splenic pDCs. (B) Expression in hemopoietic cells, including thymocytes (thym), LN B and T cells, spleen (spl) B and T cells, NK cells, immature macrophages (im mac; Mac-1+F4/80lo), mature macrophages (mat mac; Mac-1+F4/80hi), splenic pDCs and cDCs. The experiment was performed twice.

Gene expression profiles of mouse Clec9A. Real-time PCR was used to determine the mRNA expression of the Clec9A gene relative to Gapdh using primers designed from the 3′ UTR region of mClec9A. (A) Expression in lymphoid organ steady state DCs including splenic cDC subsets (DN, CD4+, CD8+), thymic cDC subsets (CD8−, CD8+) LN cDC subsets (CD8−, CD8+), dermal and Langerhans cells (LC), and in thymic and splenic pDCs. (B) Expression in hemopoietic cells, including thymocytes (thym), LN B and T cells, spleen (spl) B and T cells, NK cells, immature macrophages (im mac; Mac-1+F4/80lo), mature macrophages (mat mac; Mac-1+F4/80hi), splenic pDCs and cDCs. The experiment was performed twice.

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