Expression of caspase-8, caspase-3, and FADD protein. The basal protein expression levels of caspase-8, caspase-3, and FADD were determined by Western blot assay using whole-cell lysates from 8 patients with LGL leukemia and from 8 healthy donors. Bands indicate the position of caspase-8, caspase-3, and FADD. Western blot analysis for GAPDH was performed to confirm equal loading of total protein in each lane.