American Society of Hematology

Figure 2

$Figure 2. CD150 expression divides the CD45+EPCR+CD48− adult BM cell population into fractions differentially enriched in HSCs with differing abilities to sustain further self-renewal divisions. (A) Gates used to subdivide CD45+EPCR+CD48− adult BM cells into CD150+ and CD150− fractions as generated by FlowJo. (B) Distribution of different types of repopulating cells according to their self-renewal capacity (pie chart on the left) or differentiation (diff'n) programs displayed (bar chart on the right) from analyses of 62 mice that received a single transplantation of E-SLAM cells (top) and 28 mice that received a single translplantationof CD45+EPCR+CD48−CD150− cells (bottom). (C) Pooled results of single-cell 6-week LTC-IC assays of E-SLAM (CD150+) and CD45+EPCR+CD48−CD150− adult BM cells. (High and low SR HSCs = HSCs with durable and finite self-renewal potential, respectively; STRCs = short-term repopulating cells; and CI = confidence interval.)$

CD150 expression divides the CD45⁺EPCR⁺CD48⁻ adult BM cell population into fractions differentially enriched in HSCs with differing abilities to sustain further self-renewal divisions. (A) Gates used to subdivide CD45⁺EPCR⁺CD48⁻ adult BM cells into CD150⁺ and CD150⁻ fractions as generated by FlowJo. (B) Distribution of different types of repopulating cells according to their self-renewal capacity (pie chart on the left) or differentiation (diff'n) programs displayed (bar chart on the right) from analyses of 62 mice that received a single transplantation of E-SLAM cells (top) and 28 mice that received a single translplantationof CD45⁺EPCR⁺CD48⁻CD150⁻ cells (bottom). (C) Pooled results of single-cell 6-week LTC-IC assays of E-SLAM (CD150⁺) and CD45⁺EPCR⁺CD48⁻CD150⁻ adult BM cells. (High and low SR HSCs = HSCs with durable and finite self-renewal potential, respectively; STRCs = short-term repopulating cells; and CI = confidence interval.)

This Feature Is Available To Subscribers Only