Elevated VWF, Rhob, and Pld3 expression is consistently associated with HSCs possessing durable self-renewal potential. (A,B) Quantitative real-time PCR analyses of transcript levels from adult BM E-SLAM cells (blue bars) compared with CD45⁺EPCR⁺CD48⁻CD150⁻ cells (A, green bars) and E-SLAM cells cultured in 20 ng/mL of IL-11 supplemented with 10 ng/mL SF (B, dark purple bars) or 1 ng/mL SF (B, light purple bars). All values are normalized to Gapdh. Values shown are the mean ± SEM of values obtained in 3 to 6 independent experiments, with each measurement being derived from triplicate assays. N.D. indicates not done.